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Selective Inhibition of Gastrointestinal β-Glucuronidase by Poly(vinylbenzyl D-glucaro(1,4)lactonate). Part 2. Poly(vinylbenzyl D-Glucaro(1,4) lactonate) In vitro Inhibition Studies

Environmental Health Sciences Program, School of Public Health, University of Massachusetts, Amherst, Massachusetts 01003, USA

E.J. Calabrese

Environmental Health Sciences Program, School of Public Health, University of Massachusetts, Amherst, Massachusetts 01003, USA

In vitro inhibition studies with β-glucuronidase from purified E. coli and mouse intestinal contents indicated that the polymer, poly(vinylbenzyl D-glucaro(1,4)lactonate, is an effective β-glucuronidase inhibitor. Purified E. coli β-glucuronidase was inhibited by 99.6% with 1 77 mM D-glucaro(1,4)lactone using the polymer-inhibitor. Similarly, 95% inhibition of β-glucuronidase activity of mouse intestinal contents was obtained with 177 mM and 50% inhibition was obtained with 31.5 mM D-glucaro(1,4)lactone based on the modified polymer. The structural requirements of an effective β-glucuronidase inhibitor based on the structure of the polymer-inhibitor are also discussed.

Human & Experimental Toxicology, Vol. 13, No. 11, 759-763 (1994)
DOI: 10.1177/096032719401301104


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